In Situ Crosslinking of Highly Porous Chitosan Scaffolds for Bone Regeneration: Production Parameters and In Vitro Characterization. Issue 10 (10th July 2017)

• Record Type: Journal Article
• Authors: Kruppke, Benjamin; Farack, Jana; Sommer, Freya; Weil, Simy; Aflalo, Eliahu David; Wiesmann, Hans‐Peter; Sagi, Amir; Hanke, Thomas
• Is Part Of: Macromolecular materials and engineering. Volume 302:Issue 10(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-07-10
• Subjects: bioactivity -- bone substitute material -- chitosan -- compressive strength -- crosslinking -- scaffold; Plastics -- Periodicals; Polymers -- Periodicals; Polymerization -- Periodicals; 547.705
• DOI: 10.1002/mame.201700147
• Languages: English
• ISSNs: 1438-7492

Abstract

Abstract : Porous chitosan scaffold is produced by in situ crosslinking with glutaraldehyde, combining pore formation and crosslinking in one step. Pore formation and mechanical strength are influenced by chitosan molecular weight and degree of deacetylation, glutaraldehyde concentration, and cooling speed. The addition of calcium phosphate phases to the scaffolds allows manipulation of bioactivity, as well as the liquid change regime. Abstract : Various methods of chitosan scaffold production are reported in the literature so far. Here, in situ crosslinking with glutaraldehyde is reported for the first time. It combines pore formation and chitosan crosslinking in a single step. This combination allows incorporation of fragile molecules into 3D porous chitosan scaffolds produced by simple and gentle lyophilization. In this study, parameters of in situ crosslinking of porous chitosan scaffold formation as well as their effect on degradation and bioactivity of the scaffolds are examined. The scaffolds are characterized in the context of their prospective application as bone substitute material. The addition of calcium phosphate phases (hydroxyapatite, brushite) to the macroporous chitosan scaffolds allows manipulation of the bioactivity that is investigated by incubation in simulated body fluid (SBF). The bioactivity is significantly influenced by the modus of changing the fluid (static, daily‐, and twice‐a‐week change). Scaffolds are morphologically characterized by means of scanning electron microscopy, and the mechanical stability is tested after incubation in SBF and phosphate‐buffered saline.