In vitro basic fibroblast growth factor (bFGF) delivery using an antithrombogenic 2‐methacryloyloxyethyl phosphorylcholine (MPC) polymer coated with a micropatterned diamond‐like carbon (DLC) film. Issue 12 (23rd September 2017)

• Record Type: Journal Article
• Authors: Bito, Kenta; Hasebe, Terumitsu; Maegawa, Shunto; Maeda, Tomoki; Matsumoto, Tomohiro; Suzuki, Tetsuya; Hotta, Atsushi
• Is Part Of: Journal of biomedical materials research. Volume 105:Issue 12(2017)
• Page Start: 3384
• Page End: 3391
• Publication Date: 2017-09-23
• Subjects: DLC -- MPC -- bFGF -- drug release -- biocompatibility; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/jbm.a.36201
• Languages: English
• ISSNs: 1549-3296

Abstract

Abstract: In this study, a newly designed drug‐release platform composed of an antithrombogenic 2‐methacryloyloxyethyl phosphorylcholine (MPC) polymer was introduced, which was impregnated with basic fibroblast growth factor (bFGF) (bFGF/MPC polymer) to enhance the endothelial cell activation. The platform was also coated with an ultrathin micropatterned diamond‐like carbon (DLC) film (DLC/bFGF/MPC polymer) to precisely control the drug release rate and the cell compatibility. The resulting DLC/bFGF/MPC polymer could effectively prolong the bFGF release rate by depositing the micropatterned DLC. The number of adherent platelets on the DLC/bFGF/MPC polymer was significantly lower (about 1/14) than that on a currently used stent made of stainless steel (SUS316L), indicating the enhanced antithrombogenicity in the DLC/bFGF/MPC polymer. The proliferation of endothelial cells on the DLC/bFGF/MPC polymer and the DLC/MPC polymer (without bFGF) were also examined. It was found that the optical density of HUVEC on the DLC/bFGF/MPC polymer determined by WST‐8 assay was higher by 25%than that on the DLC/MPC polymer (without bFGF) measured after 72 h of incubation. Our results suggest that the released bFGF that contributes to the expression of other growth factors results in the early proliferation of the HUVEC on the DLC/bFGF/MPC polymer. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3384–3391, 2017.