Biological interactions between mercury and selenium in distribution and detoxification processes in mice under controlled exposure. Effects on selenoprotein. (5th March 2015)

• Record Type: Journal Article
• Authors: García-Sevillano, M.A.; Rodríguez-Moro, G.; García-Barrera, T.; Navarro, F.; Gómez-Ariza, J.L.
• Is Part Of: Chemico-biological interactions. Volume 229(2015)
• Page Start: 82
• Page End: 90
• Publication Date: 2015-03-05
• Subjects: AF affinity chromatography -- BSA bovine serum albumin -- CRMs certified reference materials -- Cys cysteine -- DNA deoxyribonucleic acid -- eGPx extracellular glutathione peroxidase -- GPx glutathione peroxidase -- GSH reduced glutathione -- HMM high molecular mass -- HPLC high performance liquid chromatography -- ICP-MS inductively coupling plasma-mass spectrometry -- IDA isotopic dilution analysis -- LC liquid chromatography -- LMM low molecular mass -- MS mass spectrometry -- MT metallothionein -- ORS octopole reaction systems -- PMSF phenylmethanesulfonyl fluoride -- RBCs red blood cells -- SeAlb selenoalbumin -- SEC size exclusion chromatography -- SelP selenoprotein P -- SeCys selenocysteine -- SUID species-unspecific isotopic dilution -- SOD superoxide dismutase -- ThxR thioredoxin reductase -- TCEP tris(2-carboxyethyl)phosphine hydrochloride -- 2D two dimensional; Mus musculus -- Mercury -- Selenium -- Metal interactions -- Metallomics -- Selenoproteins; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2015.02.001
• Languages: English
• ISSNs: 0009-2797

Abstract

Graphical abstract: Highlights: Antagonistic interactions between Hg/Se related to accumulation and detoxification. Selenoproteins may have two important roles in protecting against Hg toxicity. Selenium affects the redistribution of Hg and induces binding to inert complexes. Abstract: Antagonistic interactions between mercury (Hg) and selenium (Se), were evaluated in mouse ( Mus musculus ), as a mammalian model, in a series of controlled exposure experiments. The beneficial effect of Se against Hg toxicity involves a variety of biochemical and toxicological processes that have not been clarified yet. For this purpose, a metallomic workflow based on the use of size-exclusion chromatography (SEC) with inductively coupled plasma mass spectrometry (ICP-MS) detection was complemented with the speciation of selenoproteins and low molecular mass selenium species in serum and liver cytosolic extracts using a multidimensional approach based on SEC-AF-HPLC-ICPMS, using species-unspecific isotope dilution (SUID)-ICP-MS for selenium quantification. The results showed potential interactions between Hg/Se in organs and serum related to accumulation and detoxification processes, in addition to the effects of mercury on selenoproteins in hepatic cytosolic extracts and bloodstream when both elements are administrated at the same time. These results provide information about elements distribution, interactions and homeostasis and reveal the potential of metallomic approaches in exposure experiments.