Structural and genetic relationships of closely related O-antigens of Cronobacter spp. and Escherichia coli: C. sakazakii G2594 (serotype O4)/E. coli O103 and C. malonaticus G3864 (serotype O1)/E. coli O29. (2nd March 2015)

• Record Type: Journal Article
• Authors: Shashkov, Alexander S.; Wang, Min; Turdymuratov, Eldar M.; Hu, Shaohui; Arbatsky, Nikolay P.; Guo, Xi; Wang, Lei; Knirel, Yuriy A.
• Is Part Of: Carbohydrate research. Volume 404(2015)
• Page Start: 124
• Page End: 131
• Publication Date: 2015-03-02
• Subjects: Cronobacter sakazakii -- Cronobacter malonaticus -- O-Specific polysaccharide -- Lipopolysaccharide -- Bacterial polysaccharide structure -- O-Antigen gene cluster; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2014.11.014
• Languages: English
• ISSNs: 0008-6215

Abstract

Graphical abstract: Highlights: Structures of two related bacterial O-antigen pairs were elucidated. O-Antigens of C. sakazakii G2594 and E. coli O103 differ in the glucosylation pattern. O-Antigens of C. malonaticus G3864 and E. coli O29 differ in the O-acetylation pattern. O-Antigen gene clusters are identical within each bacterial pair. The structural differences are suggested to be due to prophage-carried genes. Abstract: O-Antigen (O-polysaccharide) variation is the basis for bacterial serotyping and is important in bacterial virulence and niche adaptation. In this work, we present structural and genetic evidences for close relationships between the O-antigens of the Cronobacter spp. and Escherichia coli . Cronobacter sakazakii G2594 (serotype O4) and Cronobacter malonaticus G3864 (serotype O1) are structurally related to those of E. coli O103 and O29, respectively, and some other members of the Enterobacteriaceae family differing in the patterns of lateral glucosylation ( C. sakazakii G2594) or O-acetylation ( C. malonaticus G3864). The O-antigen gene clusters of the corresponding Cronobacter and E. coli strains contain the same genes with high-level similarity, and the structural differences within both O-antigen pairs were suggested to be due to modification genes carried by prophages.