Structure of the O-specific polysaccharide from a marine bacterium Echinicola vietnamensis KММ 6221Т. (30th January 2015)

• Record Type: Journal Article
• Authors: Tomshich, Svetlana V.; Kokoulin, Maxim S.; Kalinovsky, Anatoliy I.; Komandrova, Nadezhda A.; Nedashkovskaya, Ol'ga I.
• Is Part Of: Carbohydrate research. Volume 402(2015)
• Page Start: 1
• Page End: 5
• Publication Date: 2015-01-30
• Subjects: O-specific polysaccharide -- Echinicola vietnamensis -- Bacterial polysaccharide structure -- Marine bacterium -- 3, 6-Dideoxy-l-xylo-hexose (Col); Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2014.05.001
• Languages: English
• ISSNs: 0008-6215

Abstract

Graphical abstract: Highlights: Structure of the O-polysaccharide of E. vietnamensis KMM 6221 T was established. The O-polysaccharide is built up of branched tetrasaccharide repeating units. 3, 6-Dideoxy-l -xylo-hexose (Col) was found in the O-polysaccharide. Abstract: The O-specific polysaccharide was isolated from the lipopolysaccharide of Echinicola vietnamensis and studied by chemical methods along with 1 H and 13 C NMR spectroscopy, including 2D 1 H, 1 H COSY, TOCSY, ROESY, 1 H, 13 C HSQC, HMBC, and H2BC experiments. It was found that the polysaccharide is built up of branched tetrasaccharide repeating units, containing 2-acetamido-2-deoxy-d -glucuronic acid (GlcNAcA), 2-acetamido-2-deoxy-d -galactose (GalNAc), andd -glucuronic acid (GlcA) with a terminal residue of 3, 6-dideoxy-l -xylo-hexose (colitose, Col) and has the following structure.