Synthesis, anti-oxidant activity, and biodegradability of a novel recombinant polysaccharide derived from chitosan and lactose. (15th March 2015)

• Record Type: Journal Article
• Authors: Guo, Ming; Ma, Yanfei; Wang, Chunge; Liu, Hongzhi; Li, Qian; Fei, Meng
• Is Part Of: Carbohydrate polymers. Volume 118(2015)
• Page Start: 218
• Page End: 223
• Publication Date: 2015-03-15
• Subjects: Chitosan -- Recombinant polysaccharide -- Lactose -- Anti-oxidation; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2014.11.027
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: The rationale of gene recombination was employed to the synthesis of recombinant polysaccharides. A novel recombinant polysaccharide (RP) based on polysaccharide–disaccharide was synthesized from oligo-chitosan and reducing lactose through Maillard reaction. Such RP exhibited remarkably improved 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity and biodegradability. Abstract: A novel recombinant polysaccharide (RP) based on polysaccharide–disaccharide was synthesized from oligo-chitosan (oligo-CS) and reducing lactose using Maillard reaction with the yield of 85.1%. Chemical structure and thermal stability of RP was characterized by Fourier transform infrared spectrum (FT-IR), solid-state nuclear magnetic resonance spectroscopy (CP/MAS 13 C-NMR), and thermo gravimetric analysis (TGA). The anti-oxidant activity of RP was preliminarily investigated by its scavenging effect on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical. Biodegradability of RP was also examined by the observation of growth status of Aspergillus niger colony. It was demonstrated that RP achieved excellent radical-scavenging efficiency (>80%) at high concentrations of DPPH and its scavenging ability was superior to that of CS, suggesting that anti-oxidant property of CS was remarkably promoted by chemical modification with reducing lactose via Maillard reaction. And biodegradation test revealed that RP had better biodegradability than CS.