The Human Colon Is More Resistant to Ischemia–reperfusion–induced Tissue Damage Than the Small Intestine: An Observational Study. Issue 2 (August 2015)

• Record Type: Journal Article
• Authors: Hundscheid, Inca H.; Grootjans, Joep; Lenaerts, Kaatje; Schellekens, Dirk H.; Derikx, Joep P.; Boonen, Bas T.; von Meyenfeldt, Maarten F.; Beets, Geerard L.; Buurman, Wim A.; Dejong, Cornelis H.
• Is Part Of: Annals of surgery. Volume 262:Issue 2(2015:Aug.)
• Publication Date: 2015-08
• Subjects: colon -- human -- ischemia -- reperfusion -- small intestine; Surgery -- Periodicals; 617.005
• DOI: 10.1097/SLA.0000000000001131
• Languages: English
• ISSNs: 0003-4932

Abstract

Abstract : Objective: Aim of this study was to draw comparisons between human colonic and jejunal ischemia–reperfusion sequelae in a human in vivo experimental model. Background: In patients, colonic ischemia–reperfusion generally has a milder course than small intestinal ischemia–reperfusion. It is unclear which pathophysiologic processes are responsible for this difference. Methods: In 10 patients undergoing colonic surgery and 10 patients undergoing pancreaticoduodenectomy, 6 cm colon or jejunum was isolated and exposed to 60 minutes ischemia followed by various reperfusion periods. Morphology (hematoxylin and eosin), apoptosis (M30), tight junctions (zonula occludens 1), and neutrophil influx (myeloperoxidase) were assessed using immunohistochemistry. Quantitative polymerase chain reaction and enzyme-linked immunosorbent assay were performed for interleukin-6 and tumor necrosis factor-α. Results: Hematoxylin and eosin staining revealed intact colonic epithelial lining, but extensive damage in jejunal villus tips after 60 minutes ischemia. After reperfusion, the colonic epithelial lining was not affected, whereas the jejunal epithelium was seriously damaged. Colonic apoptosis was limited to scattered cells in surface epithelium, whereas apoptosis was clearly observed in jejunal villi and crypts, (42 times more M30 positivity compared with colon, P < 0.01). Neutrophil influx and increased tumor necrosis factor-α mRNA expression were observed in jejunum after 30 and 120 minutes of reperfusion ( P < 0.05). Interleukin-6 mRNA expression was increased in jejunum after 120 minutes of reperfusion (3.6-fold increase, P < 0.05), whereas interleukin-6 protein expression was increased in both colon (1.5-fold increase, P < 0.05) and small intestine (1.5-fold increase, P < 0.05) after 30 and 120 minutes of reperfusion. Conclusions: Human colon is less susceptible to IR-induced tissue injury than small intestine. Abstract : Supplemental Digital Content is Available in the Text.In patients undergoing left-sided colonic surgery or pancreaticoduodenectomy for malignancy, 6-cm colon or jejunum was subjected to 45 minutes of ischemia followed by various reperfusion periods. Our data demonstrate that human colon is more resistant to ischemia–reperfusion–induced tissue injury than small intestine.