Prolonged Mouse Cardiac Graft Cold Storage via Attenuating Ischemia-Reperfusion Injury Using a New Antioxidant-Based Preservation Solution. Issue 5 (May 2016)

• Record Type: Journal Article
• Authors: Cai, Songjie; Ichimaru, Naotsugu; Zhao, Mingyi; Fujino, Masayuki; Ito, Hidenori; Ota, Urara; Nakajima, Motowo; Tanaka, Tohru; Nonomura, Norio; Li, Xiao-Kang; Takahara, Shiro
• Is Part Of: Transplantation. Volume 100:Issue 5(2016)
• Publication Date: 2016-05
• Subjects: Transplantation of organs, tissues, etc -- Periodicals; Transplantation immunology -- Periodicals; 617.95
• DOI: 10.1097/TP.0000000000001079
• Languages: English
• ISSNs: 0041-1337

Abstract

Abstract : Background: One of the major events in ischemia-reperfusion (I/R)-induced heart injury in cardiac transplantation is the generation of reactive oxygen species. We hypothesized that a novel preservation solution called SBI-SEIIKU II (SS-II) contains 3 antioxidant reagents: L-cysteine, glycine, ascorbic acid/ascorbic acid-2-phosphate magnesium, which can block the generation of reactive oxygen species to result in a prolongation of the cold storage time via attenuating I/R injury. Methods: C57BL/6CrSlc(B6) mice underwent syngeneic mice heterotopic heart transplantation, and the animals were derived into 3 groups: recipients with nonpreserved grafts (control group), recipients with grafts preserved in histidine-tryptophan-ketoglutarate (HTK) for 24 and 48 hours (HTK group), and recipients with grafts preserved in SS-II for 24 and 48 hours (SS-II group). Results: After 48 hours of preservation, there were no grafts that survived in the HTK group; however, the SS-II group had a high survival rate. After 24 hours of preservation, SS-II decreased the oxidative damage, myocardial apoptosis, and the infiltration of macrophages and neutrophils in the cardiac grafts in the early phase and suppressed the development of myocardial fibrosis in long-term grafts compared with HTK. Conclusions: The SS-II prolongs the acceptable cold storage time and protects the myocardium from I/R injury via inhibiting oxidative stress-associated damage. We believe that this novel preservation solution may be simple and safe for use in the clinical transplantation field. Abstract : Cai et al describe a novel preservation solution that incorporates 3 antioxidants: L-cysteine, glycine, and ascorbic acid/ascorbic acid-2-phosphate magnesium, which allows for prolongation of cold storage time, and attenuates ischemia-reperfusion injury, oxidative damage, myocardial apoptosis, and cellular infiltration into cardiac grafts. Supplemental digital content is available in the text.