Optimization of physical and morphological regime for improved cellulase free xylanase production by fed batch fermentation using Aspergillus niger (KP874102.1) and its application in bio-bleaching. Issue 2 (4th March 2017)

• Record Type: Journal Article
• Authors: Prasad Uday, Uma Shankar; Bandyopadhyay, Tarun Kanti; Goswami, Saswata; Bhunia, Biswanath
• Is Part Of: Bioengineered. Volume 8:Issue 2(2017)
• Page Start: 137
• Page End: 146
• Publication Date: 2017-03-04
• Subjects: bio-bleaching agent -- fermentation -- fungi -- markers -- xylanase; Biomedical engineering -- Periodicals; Biotechnology -- Periodicals; Microbiology -- Periodicals; 660.6
• DOI: 10.1080/21655979.2016.1218580
• Languages: English
• ISSNs: 2165-5987

Abstract

ABSTRACT: The physiological and morphological changes were extensively studied during fed batch fermentation using newly isolated Aspergillus niger (KP874102.1). Significantly higher xylanase production was possible through optimization of environmental stresses by fed batch process. The fed batch fermentation was carried out for improved xylanase production (2524 U) where initial xylan was kept 1.5 g/L in the production medium. However, 3 g/L of xylan with 50 mM K2 HPO4 having pH-7 was consecutively fed at 72 and 120 h of fermentation. K2 HPO4 showed significant role both the morphology of the microorganism and produces enzymes in fed batch fermentation. During feeding phase, the pH was found in the range of 6.5 to 7 which was used as marker for the fed batch process. The crude enzyme was used for the bio-bleaching of banana pulp.