MiR-143 inhibits proliferation and induces apoptosis of mammary epithelial cells in dairy goat. Issue 2 (3rd March 2016)

• Record Type: Journal Article
• Authors: Ji, Zhibin; Wang, Guizhi; Hou, Lei; Liu, Zhaohua; Wang, Jianmin; Chao, Tianle
• Is Part Of: Animal cells and systems. Volume 20:Issue 2(2016)
• Page Start: 63
• Page End: 69
• Publication Date: 2016-03-03
• Subjects: MicroRNA -- mammary epithelial cell -- flow cytometry -- hoechst33342/PI; Systems biology -- Periodicals; Biology -- Periodicals; Cytology -- Periodicals; Zoology -- Periodicals; 590
• DOI: 10.1080/19768354.2016.1165288
• Languages: English
• ISSNs: 1976-8354

Abstract

ABSTRACT: MicroRNAs are a class of post-transcriptional regulators of gene expression in multicellular organisms, which play important roles in cell fate, organ morphogenesis and carcinogenesis. In the present study, we demonstrated the critical roles of miR-143 on mammary epithelial cells of dairy goat. The test results of cell count, methylthiazolyldiphenyl-tetrazolium bromide, Hoechst33342/PI (propidium iodide) and flow cytometry showed miR-143-induced G0/G1 phase arrest, inhibited proliferation and promoted apoptosis of mammary epithelial cells; the qRT-PCR test of marker genes related to cell proliferation and apoptosis, BAX and BCL-2, supported the same conclusions. Our study presents evidence that miR-143 is an important post-transcription regulator involving in mammary cells survival, and it may have a value function in mammary gland development, lactation or involution.