Doxorubicin-Induced Gut Toxicity in Piglets Fed Bovine Milk and Colostrum. Issue 6 (December 2016)

• Record Type: Journal Article
• Authors: Shen, René L.; Rathe, Mathias; Jiang, Pingping; Pontoppidan, Peter E.L.; Heegaard, Peter M.H.; Müller, Klaus; Sangild, Per T.
• Is Part Of: Journal of pediatric gastroenterology and nutrition. Volume 63:Issue 6(2016)
• Page Start: 698
• Page End: 707
• Publication Date: 2016-12
• Subjects: bovine colostrum -- chemotherapy -- gastrointestinal toxicity -- mucositis -- porcine; Children -- Nutrition -- Periodicals; Pediatric gastroenterology -- Periodicals; Infants -- Nutrition -- Periodicals; Nutrition disorders in children -- Periodicals; Child Nutrition -- Periodicals; Digestive System -- growth & development -- Periodicals; Gastrointestinal Diseases -- Periodicals; Infant Nutrition -- Periodicals; Nutrition Disorders -- Periodicals; Child; 618.923
• DOI: 10.1097/MPG.0000000000001205
• Languages: English
• ISSNs: 0277-2116

Abstract

Abstract : Supplemental Digital Content is available in the text ABSTRACT: Objective: Chemotherapy-induced intestinal toxicity is a common adverse effect of cancer treatment. We hypothesized that a milk diet containing bovine colostrum (BC) would reduce intestinal toxicity in doxorubicin-treated piglets. Methods: "Study 1" investigated intestinal parameters 9 days after a single dose of doxorubicin (1 × 75 mg/m 2 ) in piglets fed bovine milk enriched with whey protein (BM). In "study 2, " responses to doxorubicin treatment were investigated in piglets receiving either 7 BC feedings per day (Only-BC, n = 13), 4 BC feedings (High-BC, n = 13), 2 BC feedings (Low-BC, n = 14), or no BC (only BM, n = 13). Results: Doxorubicin treatment induced clinical signs of intestinal toxicity with diarrhea and weight loss, relative to controls ( P < 0.05). White blood cells, hexose absorptive function, plasma citrulline, weights of intestine, colon, and spleen were reduced, whereas gut permeability and plasma C-reactive protein levels were increased (all P < 0.05). Limited or no effects were observed for digestive enzymes, proinflammatory cytokines, or tight-junction proteins in the intestine. Increasing BC supplementation to doxorubicin-treated piglets (study 2) had no consistent effects on plasma C-reactive protein and citrulline levels, intestinal morphology, digestive enzymes, permeability, or proinflammatory cytokines. Only-BC pigs, however, had lower diarrhea severity toward the end of the experiment ( P < 0.05 vs BM) and across the BC groups, intestinal toxicity was reduced ( P < 0.01). Conclusions: Doxorubicin-treated piglets are relevant for studying chemotherapy-induced gut toxicity. Colostrum supplementation had limited effects on doxorubicin-induced toxicity in milk-fed piglets suggesting that colostrum and a bovine milk diet enriched with whey protein provided similar protection of the developing intestine from chemotherapy-induced toxicity.