Structure of an arabinogalactan from the edible tropical fruit tamarillo (Solanum betaceum) and its antinociceptive activity. (13th February 2015)

• Record Type: Journal Article
• Authors: do Nascimento, Georgia Erdmann; Corso, Claudia Rita; de Paula Werner, Maria Fernanda; Baggio, Cristiane Hatsuko; Iacomini, Marcello; Cordeiro, Lucimara M.C.
• Is Part Of: Carbohydrate polymers. Volume 116(2015)
• Page Start: 300
• Page End: 306
• Publication Date: 2015-02-13
• Subjects: Tamarillo -- Solanum betaceum -- Type I Arabinogalactan -- Highly-methoxylated homogalacturonan -- Antinociceptive effect; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2014.03.032
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A type I arabinogalactan was found in the pulp of tamarillo fruits. A highly-methoxylated homogalacturonan was also present. The type I arabinogalactan had a main chain branched only at O -6. It was showed an antinociceptive effect on inflammatory pain models. Abstract: A structural characterization of polysaccharides obtained by aqueous extraction of ripe pulp of the edible exotic tropical fruit named tamarillo ( Solanum betaceum ) was carried out. After fractionation by freeze–thaw and α-amylase treatments, a fraction containing a mixture of highly-methoxylated homogalacturonan and of arabinogalactan was obtained. A degree of methylesterification (DE) of 71% and a degree of acetylation (DA) of 1.3% was determined by 1 H NMR spectroscopy and spectrophotometric quantification, respectively. A type I arabinogalactan was purified via Fehling precipitation and ultrafiltration through 50 kDa (cut-off) membrane. Its chemical structure was performed by sugar composition, HPSEC, methylation, carboxy-reduction and 13 C NMR spectroscopy analysis. Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.