1-[4-Fluoro-2-(2-nitrovinyl)phenyl]pyrrolidine Suppresses Toll-Like Receptor 4 Dimerization Induced by Lipopolysaccharide. Issue 3 (3rd May 2016)

• Record Type: Journal Article
• Authors: Ahn, Sang-II; Kim, Ji-Soo; Hong, Chae-Yeon; Gu, Gyo-Jeong; Shin, Hyeon-Myeong; Jeong, Hyun Jung; Koh, Kwang Oh; Mang, Joo Yang; Kim, Dae Young; Youn, Hyung-Sun
• Is Part Of: Journal of immunoassay & immunochemistry. Volume 37:Issue 3(2016)
• Page Start: 307
• Page End: 315
• Publication Date: 2016-05-03
• Subjects: toll-like receptor 4 -- 1-[4-Fluoro-2-(2-nitrovinyl)phenyl]pyrrolidine -- inflammation -- lipopolysaccharide -- dimerization -- NF-κB; Immunoassay -- Periodicals; Immunochemistry -- Periodicals; Immunoassay -- periodicals; Immunochemistry -- periodicals; 571.96405
• DOI: 10.1080/15321819.2015.1135162
• Languages: English
• ISSNs: 1532-1819

Abstract

Abstract : Toll-like receptor 4 (TLR4) recognizes LPS and triggers the activation of the myeloid differential factor 88 (MyD88)- and toll-interleukin-1 receptor domain-containing adapter, inducing interferon-β (TRIF)-dependent major downstream signaling pathways. Previously, we presented biochemical evidence that 1-[4-Fluoro-2-(2-nitrovinyl)phenyl]pyrrolidine (FPP), which was synthesized in our laboratory, inhibits NF-κB activation induced by LPS. Here, we investigated whether FPP modulates the TLR4 downstream signaling pathways and what anti-inflammatory target in TLR4 signaling is regulated by FPP. FPP inhibited LPS-induced NF-κB activation by targeting TLR4 dimerization. These results suggest that FPP can modulate the TLR4 signaling pathway at the receptor level to decrease inflammatory gene expression.