Dendritic cell type-specific HIV-1 activation in effector T cells: implications for latent HIV-1 reservoir establishment. (1st June 2015)

• Record Type: Journal Article
• Authors: van der Sluis, Renée M.; van Capel, Toni M.M.; Speijer, Dave; Sanders, Rogier W.; Berkhout, Ben; de Jong, Esther C.; Jeeninga, Rienk E.; van Montfort, Thijs
• Is Part Of: AIDS. Volume 29:Number 9(2015)
• Publication Date: 2015-06-01
• Subjects: dendritic cell disruption of HIV-1 latency -- dendritic cell subset-specific purging -- dendritic cell–T-cell interactions -- HIV-1 -- HIV-1 latency establishment -- reversion of HIV-1 latency; AIDS (Disease) -- Periodicals; Acquired Immunodeficiency Syndrome; AIDS (Disease); Periodicals; Periodicals; 616.9792005
• DOI: 10.1097/QAD.0000000000000637
• Languages: English
• ISSNs: 0269-9370

Abstract

Abstract : Background: Latent HIV type I (HIV-1) infections can frequently occur in short-lived proliferating effector T lymphocytes. These latently infected cells could revert into resting T lymphocytes and thereby contribute to the establishment of the long-lived viral reservoir. Monocyte-derived dendritic cells can revert latency in effector T cells in vitro . Methods: Here we investigated the latency activation properties of tissue-specific immune cells, including a large panel of dendritic cell subsets, to explore in which body compartments effector T cells are most likely to maintain latent HIV-1 provirus and thus potentially contribute to the long-lived reservoir. Results: Our results demonstrate that blood or genital tract dendritic cells do not activate latent provirus in effector T cells, whereas gut or lymphoid dendritic cells induce virus production from latently infected effector T cells in our in-vitro model for latency. Toll-like receptor 3-induced interferon production by myeloid dendritic cells abolished the dendritic cells' ability to induce viral gene expression. Conclusions: In this study, we show that HIV-1 provirus residing in effector T cells is activated from latency by tissue-specific dendritic cell subsets and other immune cells with remarkably different efficiencies. Our new assay system points to an important, neglected aspect of HIV-1 research: the ability of other immune cells, especially dendritic cells, to differentially affect latency establishment as well as virus reactivation. Abstract : Supplemental Digital Content is available in the text