Tissue microarray advanced techniques for sampling donor blocks with limited tissues – introduction to 'the radical-TMA'. (March 2014)

• Record Type: Journal Article
• Authors: Jensen, Thom; Cessna, Melissa; Miller, Dylan; Hansen, Janet
• Is Part Of: Journal of histotechnology. Volume 37:Number 1(2014)
• Page Start: 9
• Page End: 13
• Publication Date: 2014-03
• Subjects: Antibody optimizing, -- Arraymold, -- Pathology validation, -- Radical-TMA, -- Tissue microarray; Histology -- Periodicals; Cytology -- Periodicals; 611.018
• DOI: 10.1179/2046023613Y.0000000035
• Languages: English
• ISSNs: 0147-8885

Abstract

Abstract : Tissue microarrays (TMAs) are becoming more common in pathology. 4 –6, 11, 12 With the costs of validating instruments or optimizing antibodies and control tissues on the rise, the best way to cut costs would be to use TMAs. Good laboratory techniques for constructing TMAs are essential for the technician. Tissue samples are extremely valuable and often depleted because of specialized histologic testing. The costs saved using TMAs outweigh the expense of purchasing a TMA instrument. A sound method for optimizing tissue samples for TMA construction would be beneficial to the medical and research community. Advanced or radical-tissue microarray (R-TMA) techniques require only minute tissue samples that were not amenable to TMA construction in the past.