Contribution of the csgA and bcsA genes to Salmonella enterica serovar Pullorum biofilm formation and virulence. Issue 5 (3rd September 2017)

• Record Type: Journal Article
• Authors: El Hag, Muhanad; Feng, Zheng; Su, Yangyang; Wang, Xiao; Yassin, Afrah; Chen, Sujuan; Peng, Daxin; Liu, Xiufan
• Is Part Of: Avian pathology. Volume 46:Issue 5(2017)
• Page Start: 541
• Page End: 547
• Publication Date: 2017-09-03
• Subjects: Salmonella enterica serovar Pullorum -- biofilm -- csgA -- bcsA -- mutant -- pathogenicity; Poultry -- Diseases -- Periodicals; Birds -- Diseases -- Periodicals; 571.918
• DOI: 10.1080/03079457.2017.1324198
• Languages: English
• ISSNs: 0307-9457

Abstract

ABSTRACT: Salmonella biofilm formation is important to environmental stress resistance and virulence. However, the roles of the csgA and bcsA genes, which affect curli protein and cellulose production, respectively, in Salmonella enterica serovar Pullorum, are unknown. Here we constructed deletions in the csgA and bcsA genes in S. enterica serovar Pullorum strain S6702 and evaluated several aspects of biofilm formation and virulence. Δ csgA showed decreased production of curli fimbriae, while Δ bcsA had reduced cellulose production. Both mutants had a reduced ability to form biofilms. Δ csgA was reduced in adhesion and invasion to HeLa cells and exhibited decreased intracellular proliferation in HD11 macrophages. Δ bcsA exhibited increased proliferation in HD11 cells and replicated better in chicken spleens, as compared to the wild-type strain. Δ csgA virulence was attenuated in assays involving oral challenge of one-day-old chickens.