Ionic liquid/ultrasound pretreatment and in situ enzymatic saccharification of bagasse using biocompatible cholinium ionic liquid. (January 2015)

• Record Type: Journal Article
• Authors: Ninomiya, Kazuaki; Kohori, Asami; Tatsumi, Mai; Osawa, Koji; Endo, Takatsugu; Kakuchi, Ryohei; Ogino, Chiaki; Shimizu, Nobuaki; Takahashi, Kenji
• Is Part Of: Bioresource technology. Volume 176(2015)
• Page Start: 169
• Page End: 174
• Publication Date: 2015-01
• Subjects: Cholinium -- Ionic liquid -- Pretreatment -- In situ saccharification -- Lignocellulose; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2014.11.038
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Choline acetate (ChOAc) pretreated bagasse as imidazolium ionic liquid (EmimOAc). ChOAc showed less inhibitory effect on Ctec2 cellulase than EmimOAc. ChOAc showed better performance of in situ enzymatic hydrolysis than EmimOAc. Afterward, glucose/xylose solution free from ChOAc was recovered by electrodialysis. Abstract: Choline acetate (ChOAc), a cholinium ionic liquid (IL), showed almost the same bagasse pretreatment capability as 1-ethyl-3-methylimidazolium acetate (EmimOAc), a conventional imidazolium IL used for biomass pretreatment. Moreover, ChOAc showed less of an inhibitory effect on cellulase than EmimOAc. Thus, ChOAc was used for IL/ultrasound-assisted pretreatment and in situ enzymatic saccharification, where IL was not washed out from the pretreated bagasse but diluted with the addition of a buffer solution. When in situ saccharification was performed for 48 h in the presence of 10% ChOAc, the cellulose and hemicellulose saccharification percentages were 80% and 72%, respectively. When ChOAc was increased to 20%, the saccharification percentages were 72% and 53%, respectively. However, the values were just 28% and 2%, respectively, in case of 20% EmimOAc. A glucose/xylose solution free from IL and ChOAc aqueous solution without these sugars could be recovered separately by electrodialysis of the hydrolysate of in situ saccharification.