Anti-biofilm mechanisms of 3, 5-di-tert-butylphenol against clinically relevant fungal pathogens. Issue 9 (20th October 2016)

• Record Type: Journal Article
• Authors: Rathna, Janarthanam; Bakkiyaraj, Dhamodharan; Pandian, Shunmugiah Karutha
• Is Part Of: Biofouling. Volume 32:Issue 9(2016)
• Page Start: 979
• Page End: 993
• Publication Date: 2016-10-20
• Subjects: Candida albicans -- Candida tropicalis -- Pleurotus florida -- 3, 5-di-tert-butylphenol -- cell wall integrity -- ROS accumulation; Marine fouling organisms -- Periodicals; Materials -- Biodeterioration -- Periodicals; Fouling -- Periodicals; Biodegradation -- Periodicals; Environmental Microbiology -- Periodicals; Water Microbiology -- Periodicals; 576.165
• DOI: 10.1080/08927014.2016.1216103
• Languages: English
• ISSNs: 0892-7014

Abstract

Abstract: The methanolic extract (PFME) of Pleurotus florida was assessed for anti-biofilm activity against Candida species . 3, 5-Di-tert-butylphenol (3, 5-DTB) was identified as the major antifungal constituent in PFME. In its pure form 3, 5-DTB inhibits, disrupts, and reduces the viability of biofilm cells as seen from scanning electron and confocal microscopy studies. Microscopic studies and propidium iodide uptake assays confirmed that 3, 5-DTB damages the cell membrane of Candida cells. In addition, 3, 5-DTB induces accumulation of reactive oxygen species (ROS) which contribute to its pronounced anti-biofilm activity. The results of the present study show that 3, 5-DTB exhibits combined anti-biofilm and conventional fungicidal activity against Candida species and elucidate the underlying mechanisms.