N-3 fatty acids reduce plasma 20-hydroxyeicosatetraenoic acid and blood pressure in patients with chronic kidney disease. Issue 9 (September 2015)

• Record Type: Journal Article
• Authors: Barden, Anne E.; Burke, Valerie; Mas, Emilie; Beilin, Lawrence J.; Puddey, Ian B.; Watts, Gerald F.; Irish, Ashley B.; Mori, Trevor A.
• Is Part Of: Journal of hypertension. Volume 33:Issue 9(2015:Sep.)
• Publication Date: 2015-09
• Subjects: 20-hydroxyeicosatetraenoic acid -- chronic kidney disease -- F2-isoprostanes -- n-3 fatty acid supplementation; Hypertension -- Periodicals; Hypertension -- Periodicals; 616.132005
• DOI: 10.1097/HJH.0000000000000621
• Languages: English
• ISSNs: 1473-5598

Abstract

Abstract : Background: Metabolism of arachidonic acid by cytochrome P450 ω-hydroxylase leads to the formation of 20-hydroxyeicosatetraenoic acid (20-HETE) that regulates vascular function, sodium homeostasis and blood pressure (BP). Supplementation with n-3 fatty acids is known to alter arachidonic acid metabolism and reduce the formation of the lipid peroxidation products F2 -isoprostanes, but the effect of n-3 fatty acids on 20-HETE has not been studied. Method: We previously reported a significant effect of n-3 fatty acids but not coenzyme Q10 (CoQ) to reduce BP in a double-blind, placebo-controlled intervention, wherein patients with chronic kidney disease (CKD) were randomized to n-3 fatty acids (4 g), CoQ (200 mg), both supplements or control (4 g olive oil), daily for 8 weeks. This study examined the effect of n-3 fatty acids on plasma and urinary 20-HETE in the same study, as well as plasma and urinary F2 -isoprostanes, and relate these to changes in BP. Results: Seventy-four patients completed the 8-week intervention. n-3 fatty acids but not CoQ significantly reduced plasma 20-HETE ( P = 0.001) and F2 -isoprostanes ( P < 0.001). In regression models adjusted for BP at baseline, postintervention plasma 20-HETE was a significant predictor of the fall in SBP ( P < 0.0001) and DBP ( P < 0.0001) after n-3 fatty acids. Conclusion: This is the first report that n-3 fatty acid supplementation reduces plasma 20-HETE in humans and that this associates with reduced BP. These results provide a plausible mechanism for the reduction in BP observed in patients with CKD following n-3 fatty acid supplementation.