Supramolecular gel electrophoresis of large DNA fragments. Issue 20 (25th August 2017)

• Record Type: Journal Article
• Authors: Tazawa, Shohei; Kobayashi, Kazuhiro; Oyoshi, Takanori; Yamanaka, Masamichi
• Other Names: Flanagan Lisa A. guestEditor.; Labeed Fatima H. guestEditor.; Lapizco‐Encinas Blanca H. guestEditor.
• Is Part Of: Electrophoresis. Volume 38:Issue 20(2017)
• Page Start: 2662
• Page End: 2665
• Publication Date: 2017-08-25
• Subjects: DNA -- Electrophoresis -- Hydrogel -- Self‐assembly -- Supramolecular chemistry; Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201700223
• Languages: English
• ISSNs: 0173-0835

Abstract

Abstract: Pulsed‐field gel electrophoresis is a frequent technique used to separate exceptionally large DNA fragments. In a typical continuous field electrophoresis, it is challenging to separate DNA fragments larger than 20 kbp because they migrate at a comparable rate. To overcome this challenge, it is necessary to develop a novel matrix for the electrophoresis. Here, we describe the electrophoresis of large DNA fragments up to 166 kbp using a supramolecular gel matrix and a typical continuous field electrophoresis system. C 3 ‐symmetric tris‐urea self‐assembled into a supramolecular hydrogel in tris‐boric acid‐EDTA buffer, a typical buffer for DNA electrophoresis, and the supramolecular hydrogel was used as a matrix for electrophoresis to separate large DNA fragments. Three types of DNA marker, the λ‐Hind III digest (2 to 23 kbp), Lambda DNA‐Mono Cut Mix (10 to 49 kbp), and Marker 7 GT (10 to 165 kbp), were analyzed in this study. Large DNA fragments of greater than 100 kbp showed distinct mobility using a typical continuous field electrophoresis system.