An HPLC method for simultaneous quantitative determination of seven secoiridoid glucosides separated from the roots of Ilex pubescens. (30th May 2017)

• Record Type: Journal Article
• Authors: Zhang, Yang; Xiong, Huan; Bi, Jiayi; Zhao, Guiyun; Yang, Yi; Chen, Xue; Li, Yuncong; Zhang, Chongyu; Zhang, Guogang
• Is Part Of: Biomedical chromatography. Volume 31:Number 11(2017:Nov.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-05-30
• Subjects: determination -- Ilex pubescens -- secoiridoid glucosides; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3995
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: A simple and specific high‐performance liquid chromatographic method has been developed and validated to simultaneously determine seven secoiridoid glucosides for the first time. Three of them were separated from the ethanolic extract of the roots of Ilex pubescens for the first time, namely nuezhenide A, ligusides B and oleonuezhenide. In quantitative analysis, all of the calibration curves showed good linear regression ( r > 0.999) within the tested ranges, and the mean recoveries of three different concentrations ranged from 97.6 to 101.2%. The limit of detection and limit of quantification were <4.18 and 11.63 ng mL −1, respectively. The relative standard deviation for repeatability and the precision of seven analytes were <3.4 and 1.9%, respectively. The established method was successfully applied to simultaneous determination of seven secoiridoid glucosides in 11 batches of samples collected from different habitats in China.