Modification of ligands for serum albumin on polyethyleneimine to stabilize polyplexes in gene delivery. Issue 13 (2nd September 2017)

• Record Type: Journal Article
• Authors: Nakamura, Yuta; Sato, Hikari; Nobori, Takanobu; Matsumoto, Hotaru; Toyama, Shoko; Shuno, Tomohiro; Kishimura, Akihiro; Mori, Takeshi; Katayama, Yoshiki
• Is Part Of: Journal of biomaterials science. Volume 28:Issue 13(2017)
• Page Start: 1382
• Page End: 1393
• Publication Date: 2017-09-02
• Subjects: Gene delivery -- polyethyleneimine -- serum albumin -- colloidal stability -- ternary complex; Polymers -- Biocompatibility -- Periodicals; Biomedical materials -- Periodicals; 572.33
• DOI: 10.1080/09205063.2017.1328730
• Languages: English
• ISSNs: 0920-5063

Abstract

Abstract: In this work, we have developed a new technique to stabilize a ternary complex composed of plasmid DNA, a linear polyethyleneimine (LPEI) and serum albumin. A stearoyl group was conjugated to LPEI as a specific ligand for serum albumin. The resultant ternary complex has excellent stability in physiological saline conditions, maintaining its initial diameter and preventing aggregation of red blood cells. The ternary complex has equivalent transfection ability to and significantly lower cytotoxicity than unmodified LPEI. Therefore, the ternary complex is potentially useful as a new gene carrier, possessing high blood stability.