Bioprocess development for the production of sonorensin by Bacillus sonorensis MT93 and its application as a food preservative. (January 2015)

• Record Type: Journal Article
• Authors: Chopra, Lipsy; Singh, Gurdeep; Jena, Kautilya Kumar; Verma, Himanshu; Sahoo, Debendra K.
• Is Part Of: Bioresource technology. Volume 175(2015)
• Page Start: 358
• Page End: 366
• Publication Date: 2015-01
• Subjects: Bacillus sonorensis -- Sonorensin -- Optimization -- Volumetric oxygen transfer coefficient -- Biopreservative; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2014.10.105
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Optimization of sonorensin production by RSM led to 15-fold increase in its yield. Sonorensin production in a bioreactor validated optimized medium composition. Sonorensin production was influenced by medium composition and process parameters. Sonorensin showed its potential as a biopreservative. Abstract: Media composition and environmental conditions were optimized using statistical tools, Plackett Burman design and response surface methodology, to maximize the yield of a bacteriocin, named as sonorensin, from a new marine isolate Bacillus sonorensis MT93 showing broad spectrum of antimicrobial activity. Under optimized conditions, MT93 produced 15-fold higher yield of sonorensin compared to that under initial fermentation conditions. As oxygen supply is a critical parameter controlling growth and product formation in aerobic bioprocesses and used as a parameter for bioprocess scale up, the effects of oxygen transfer, in terms of volumetric oxygen transfer coefficient ( k L a ), on production of sonorensin was investigated using optimized medium composition in a bioreactor. Studies on effectiveness of sonorensin against Staphylococcus aureus and Listeria monocytogenes in fruit juice and as a preservative in pasteurized milk demonstrated its potential as a biopreservative in fruit products and shelf life extender of the pasteurized milk.