A pH-responsive folate conjugated magnetic nanoparticle for targeted chemo-thermal therapy and MRI diagnosis. Issue 6 (21st December 2015)

• Record Type: Journal Article
• Authors: Gupta, Jagriti; Mohapatra, Jeotikanta; Bhargava, Parag; Bahadur, D.
• Is Part Of: Dalton transactions. Volume 45:Issue 6(2016)
• Page Start: 2454
• Page End: 2461
• Publication Date: 2015-12-21
• Subjects: Chemistry, Inorganic -- Periodicals; Chemistry, Physical and theoretical -- Periodicals; Chemistry, Inorganic -- Periodicals; 546.05
• DOI: 10.1039/c5dt04135j
• Languages: English
• ISSNs: 1477-9226

Abstract

Abstract : Schematic representation of chemo and thermal therapy of folate conjugated magnetic nanoparticles (FA-MNPs) against cancer cells. Abstract : Polyacrylic acid functionalized Fe3 O4 nanoparticles (PAA-MNPs) of average size of 10 nm are prepared by a simple soft chemical approach. These PAA-MNPs are conjugated with folic acid through peptide bonding between the carboxylic group on the surface of PAA-MNPs and the amine group of folic acid. The good colloidal stability of FA conjugated MNPs makes it a promising candidate for targeted drug delivery, hyperthermia and as a MRI contrast agent with a transverse relaxivity R 2 value of 105 mM −1 s −1 . Folic acid conjugated magnetic nanoparticles (FA-MNPs) achieved ∼95% loading efficiency of doxorubicin (DOX) which could be due to strong electrostatic interaction of highly negatively charged FA-MNPs and the positively charged DOX. The drug release study shows a pH-dependent behavior and is higher in acidic pH (4.3 and 5.6) as compared to the physiological pH (7.3). Flow cytometry and confocal microscopic image analysis reveal that around 75–80% of HeLa cells undergo apoptosis due to DNA disintegration upon incubation with DOX-MNPs for 24 h. DOX-MNPs exhibit the synergistic effect due to the combination of DOX induced apoptosis and magnetic hyperthermia treatment (MHT) which enhance the cell death ∼95.0%. Thus, this system may serve as a potential pH sensitive nanocarrier for synergistic chemo-thermal therapy as well as a possible MRI contrast agent.