Neuromodulatory properties of fluorescent carbon dots: Effect on exocytotic release, uptake and ambient level of glutamate and GABA in brain nerve terminals. (February 2015)

• Record Type: Journal Article
• Authors: Borisova, Tatiana; Nazarova, Anastasia; Dekaliuk, Mariia; Krisanova, Natalia; Pozdnyakova, Natalia; Borysov, Arsenii; Sivko, Roman; Demchenko, Alexander P.
• Is Part Of: International journal of biochemistry & cell biology. Volume 59(2015:Feb.)
• Page Start: 203
• Page End: 215
• Publication Date: 2015-02
• Subjects: Carbon dots -- Neurotransmitters -- Exocytosis -- Na+-dependent uptake -- Brain nerve terminals; Biochemistry -- Periodicals; Cytology -- Periodicals; Biochemistry -- Periodicals; Cell Biology -- Periodicals; Biochimie -- Périodiques; Cytologie -- Périodiques; Biochimie; Cytologie; Biochemistry; Cytology; Ressource Internet (Descripteur de forme); Périodique électronique (Descripteur de forme); Periodicals; 572.05
• DOI: 10.1016/j.biocel.2014.11.016
• Languages: English
• ISSNs: 1357-2725

Abstract

Graphical abstract: Abstract: Carbon dots (C-dots), a recently discovered class of fluorescent nano-sized particles with pure carbon core, have great bioanalytical potential. Neuroactive properties of fluorescent C-dots obtained from β-alanine by microwave heating were assessed based on the analysis of their effects on the key characteristics of GABA- and glutamatergic neurotransmission in isolated rat brain nerve terminals. It was found that C-dots (40–800 μg/ml) in dose-dependent manner: (1) decreased exocytotic release of [ 3 H]GABA andl -[ 14 C]glutamate; (2) reduced acidification of synaptic vesicles; (3) attenuated the initial velocity of Na + -dependent transporter-mediated uptake of [ 3 H]GABA andl -[ 14 C]glutamate; (4) increased the ambient level of the neurotransmitters, nevertheless (5) did not change significantly the potential of the plasma membrane of nerve terminals. Almost complete suppression of exocytotic release of the neurotransmitters was caused by C-dots at a concentration of 800 μg/ml. Fluorescent and neuromodulatory features combined in C-dots create base for their potential usage for labeling and visualization of key processes in nerve terminals, and also in theranostics. In addition, natural presence of carbon-containing nanoparticles in the human food chain and in the air may provoke the development of neurologic consequences.