Cysteine‐rich buccal gland protein suppressed the proliferation, migration and invasion of hela cells through akt pathway. Issue 11 (25th September 2017)

• Record Type: Journal Article
• Authors: Han, Jianmei; Liu, Yu; Jiang, Qi; Xiao, Rong
• Is Part Of: IUBMB life. Volume 69:Issue 11(2017)
• Page Start: 856
• Page End: 866
• Publication Date: 2017-09-25
• Subjects: cysteine‐rich buccal gland protein -- cysteine‐rich secretory proteins -- Hela cells -- voltage‐gated sodium channels -- Lampetra japonica; Biochemistry -- Periodicals; Molecular biology -- Periodicals; 572.8
• DOI: 10.1002/iub.1680
• Languages: English
• ISSNs: 1521-6543

Abstract

Abstract: Cysteine‐rich buccal gland protein (CRBGP) as a member of cysteine‐rich secretory proteins (CRISPs) superfamily was isolated from the buccal glands of Lampetra japonica, the blood suckers in the marine. Previous studies showed CRBGP could suppress angiogenesis probably due to its ion channel blocking activity. Whether CRBGP could also affect the activity of tumor cells has not been reported yet. In this study, CRBGP suppressed the proliferation of Hela cells with an IC50 of 6.7 μM by inducing apoptosis. Both microscopic observation and Western blot indicated that CRBGP was able to induce the nuclei shrinking, downregulate the protein level of BCL2 and caspase 3 as well as upregulate the level of BAX in Hela cells, suggested that CRBGP might induce apoptosis of Hela cells in a mitochondrial‐dependent pathway. Furthermore, CRBGP could disturb F‐actin organization, which would finally cause the Hela cells to lose their shape and to lessen their abilities on adhesion, migration and invasion. Finally, CRBGP was shown to reduce the phosphorylation level of Akt, which indicated that CRBGP might inhibit the proliferation and metastasis of Hela cells through Akt pathway. CRBGP, as a voltage‐gated sodium channel blocker, also possesses the anti‐tumor abilities which provided information on the effects and action manner of the other CRISPs. © 2017 IUBMB Life, 69(11):856–866, 2017