Supramolecular conjugated polymer materials for organelle imaging in living cells. (9th May 2017)

• Record Type: Journal Article
• Authors: Hu, Rong; Li, Shengliang; Lu, Huan; Liu, Libing; Lv, Fengting; Wang, Shu
• Is Part Of: Materials chemistry frontiers. Volume 1:Number 9(2017)
• Page Start: 1768
• Page End: 1772
• Publication Date: 2017-05-09
• Subjects: Materials science -- Periodicals; Chemistry -- Periodicals; 540
• DOI: 10.1039/c7qm00105c
• Languages: English
• ISSNs: 2052-1529

Abstract

Abstract : A novel supramolecular system based on conjugated polymers is developed to realize specific targeting and imaging of organelles in living cells. Abstract : A novel supramolecular system based on conjugated polymers was developed to realize specific targeting and imaging of organelles in living cells. The carboxyl group-modified polythiophene derivative (PT) forms a complex with six histidine equipped Tat peptide via Ni 2+ ion chelation, hydrogen bonds and electrostatic interactions (PT/Ni 2+ /Tat-His6 ). The complex can reassemble with RFP-His10 due to the relatively high affinity of Ni 2+ towards His10 -tag compared to His6 -tag. Thus, the PT/Ni 2+ /Tat-His6 complex could selectively label and image organelles expressing His10 -tag in living cells with high stability.