Synthesis, characterization, and DNA binding studies of the hematoxylin-Zn(II) complex. Issue 5 (4th May 2017)

• Record Type: Journal Article
• Authors: Xiong, Xiao-Li; You, Chao; Xue, Kang; Huang, Jian-Hang; Wang, Chun-Ling
• Is Part Of: Inorganic and nano-metal chemistry. Volume 47:Issue 5(2017)
• Page Start: 794
• Page End: 799
• Publication Date: 2017-05-04
• Subjects: Hematoxylin-Zn(II) complex -- herring sperm DNA -- binding mechanism -- acridine orange; Inorganic compounds -- Synthesis -- Periodicals; Nanostructured materials -- Periodicals; Reactivity (Chemistry) -- Periodicals; Inorganic compounds -- Synthesis; Nanostructured materials; Reactivity (Chemistry); Periodicals; 546
• DOI: 10.1080/15533174.2016.1216453
• Languages: English
• ISSNs: 2470-1556

Abstract

ABSTRACT: The binding mechanism of Hematoxylin-Zn(II) complex with Herring sperm DNA has been investigated using UV–vis and fluorescence spectroscopies and viscosity measurement. With the increase in the concentration of complex, the viscosity of DNA decreased, and the binding mode is more detailed: there is partially intercalate binding. Furthermore, besides the intercalate binding, the existence of groove binding was confirmed through the ionic strength experiment in Scatchard analysis. Thermodynamic studies provided some thermodynamic parameters: Δr H m Θ = 1.43 × 10 5 J mol −1, Δr S m Θ = 576.14 J mol −1, Δr G m Θ 303K = −3.16 × 10 4 J mol −1, they illustrated that the binding mechanism between HE-Zn(II) complex and hsDNA was driven mainly by entropy.