Aluminium glycinate functionalized silica nanoparticles for highly specific separation of phosphoproteins. Issue 31 (21st July 2015)

• Record Type: Journal Article
• Authors: Liu, Wei; Zheng, Jiangnan; Li, Shihua; Wang, Ruirui; Lin, Zian; Yang, Huanghao
• Is Part Of: Journal of materials chemistry. Volume 3:Issue 31(2015)
• Page Start: 6528
• Page End: 6535
• Publication Date: 2015-07-21
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c5tb01055a
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : Aluminium glycinate functionalized silica nanoparticles were synthesized by a facile approach and successfully applied for the specific capture of phosphoproteins from a complex sample. Abstract : Selective separation of intact phosphoproteins from complex biological samples is essential for ongoing, top-down phosphoproteomics but challenges still remain. Herein, aluminium glycinate functionalized silica nanoparticles (denoted as AGNP) were synthesized by a facile approach and applied for the specific capture of phosphoproteins. The selectivity and binding capacity of AGNP were evaluated using caseins (α-casein and β-casein) as phosphoproteins and bovine haemoglobin, bovine serum albumin, horseradish peroxidase, myoglobin and lysozyme as nonphosphoproteins. The results indicated that AGNP showed high binding capacity and selectivity for phosphoproteins (α-casein 1190 mg g −1 and β-casein 1060 mg g −1 ). In addition, AGNP was used to selectively capture and enrich phosphoproteins from protein mixtures and drinking milk samples. The good results demonstrate the potential of AGNP in phosphoproteomics analysis.