Water soluble fluorophore-carbazole–Au–DNA nanohybrid: enhanced two-photon absorption for living cell imaging application. Issue 114 (3rd November 2015)

• Record Type: Journal Article
• Authors: Zhu, Shu-Juan; Kong, Lin; Wang, Hui; Tian, Yu-Peng; Li, Sheng-Li; Xue, Zhao-Ming; Xu, Xian-Yun; Yang, Jia-Xiang
• Is Part Of: RSC advances. Volume 5:Issue 114(2015)
• Page Start: 94446
• Page End: 94455
• Publication Date: 2015-11-03
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c5ra13791h
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Water solubleL –Au–DNA nanohybrid exhibits enhanced 2PA properties that can widely and evenly uptake into the lysosome of HepG2 cells. Abstract : A type of water-soluble Au based nanohybrid is constructed through a D–π–A (D = donor, A = acceptor) fluorophore-carbazole derivative (abbreviated asL ) coupled with gold nanoparticles (NPs) under the guidance of calf thymus DNA (ct-DNA). The coupling interactions between components at the interface increase the strength of donor–acceptor pair within the nanohybrid, which results in an energy transfer process and further brings about a dramatic blue-shifted single-photon absorption (about 53 nm from 450 nm forL to 397 nm for the hybrid) and fluorescence (∼77 nm blue-shifted), decreased FL lifetime (1.65 ns forL, 0.53 ns for the hybrid), and blue-shifted two-photon excited fluorescence (2PEF, 638 nm forL, 574 nm for the hybrid). The coupling effect amongst the nanohybrid also leads to enhancement of nonlinear optical properties, including the two-photon absorption (TPA) cross section ( δ, 137.60 GM forL, 651.07 GM for the hybrid) and two-photon absorption coefficient ( β, from 0.003669 cm GW −1 to 0.01401 cm GW −1 ). The two-photon fluorescence and good water solubility of the hybrid have proven to be potentially useful for two-photon microscopy imaging in living cells such as HepG2.