Preparative enrichment and purification of nevadensin from Lysionotus pauciflorus using macroporous resins. Issue 2 (22nd January 2016)

• Record Type: Journal Article
• Authors: Wen, Yaya; Du, Hongjian; Tu, Yanbei; Luo, Wei; Li, Qin; Zhu, Chao; Li, Yanfang; Liang, Bing
• Is Part Of: Separation science and technology. Volume 51:Issue 2(2016)
• Page Start: 339
• Page End: 347
• Publication Date: 2016-01-22
• Subjects: Enrichment -- Lysionotus pauciflorus -- macroporous resins -- nevadensin; Separation (Technology) -- Periodicals; 660.284205
• DOI: 10.1080/01496395.2015.1085066
• Languages: English
• ISSNs: 0149-6395

Abstract

Abstract: An efficient method using macroporous adsorption resins for preparative enrichment and purification of nevadensin from Lysionotus pauciflorus Maxim was developed. The results indicated that non-polar HPD-100 resin offered the best adsorption and desorption performance, its adsorption data were well-fitted to the Freundlich isotherm and the pseudo-second-order kinetics model. Dynamic adsorption and desorption experiments have been investigated for optimization of chromatographic parameters. Through one cycle of dynamic adsorption/desorption, the purity of nevadensin in the extract, increased about 8.82-fold from 8.58% to 75.70%, with a recovery yield of 69.90%. The results suggested that HPD-100 resin can separate nevadensin effectively from plant material.