Copper-doped borosilicate bioactive glass scaffolds with improved angiogenic and osteogenic capacity for repairing osseous defects. (1st March 2015)

• Record Type: Journal Article
• Authors: Zhao, Shichang; Wang, Hui; Zhang, Yadong; Huang, Wenhai; Rahaman, Mohamed N.; Liu, Zhongtang; Wang, Deping; Zhang, Changqing
• Is Part Of: Acta biomaterialia. Volume 14(2015)
• Page Start: 185
• Page End: 196
• Publication Date: 2015-03-01
• Subjects: Copper-doped bioactive glass -- Scaffold -- Angiogenesis -- Osteogenesis -- Stem cells; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1016/j.actbio.2014.12.010
• Languages: English
• ISSNs: 1742-7061

Abstract

Graphical abstract: Abstract: There is growing interest in the use of synthetic biomaterials to deliver inorganic ions that are known to stimulate angiogenesis and osteogenesis in vivo. In the present study, we investigated the effects of varying amounts of copper in a bioactive glass on the response of human bone marrow-derived mesenchymal stem cells (hBMSCs) in vitro and on blood vessel formation and bone regeneration in rat calvarial defects in vivo. Porous scaffolds of a borosilicate bioactive glass (composition 6Na2 O, 8K2 O, 8MgO, 22CaO, 36B2 O3, 18SiO2, 2P2 O5, mol.%) doped with 0.5, 1.0 and 3.0 wt.% CuO were created using a foam replication method. When immersed in simulated body fluid, the scaffolds released Cu ions into the medium and converted to hydroxyapatite. At the concentrations used, the Cu in the glass was not toxic to the hBMSCs cultured on the scaffolds in vitro. The alkaline phosphatase activity of the hBMSCs and the expression levels of angiogenic-related genes (vascular endothelial growth factor and basic fibroblast growth factor) and osteogenic-related genes (runt-related transcription factor 2, bone morphogenetic protein-2 and osteopontin) increased significantly with increasing amount of Cu in the glass. When implanted in rat calvarial defects in vivo, the scaffolds (3 wt.% CuO) significantly enhanced both blood vessel formation and bone regeneration in the defects at 8 weeks post-implantation. These results show that doping bioactive glass implants with Cu is a promising approach for enhancing angiogenesis and osteogenesis in the healing of osseous defects.