Injectable silk-polyethylene glycol hydrogels. (15th January 2015)

• Record Type: Journal Article
• Authors: Wang, Xiaoqin; Partlow, Benjamin; Liu, Jian; Zheng, Zhaozhu; Su, Bo; Wang, Yansong; Kaplan, David L.
• Is Part Of: Acta biomaterialia. Volume 12(2015)
• Page Start: 51
• Page End: 61
• Publication Date: 2015-01-15
• Subjects: Silk -- Polyethylene glycol -- Hydrogel -- Injectable -- Stem cells; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1016/j.actbio.2014.10.027
• Languages: English
• ISSNs: 1742-7061

Abstract

Graphical abstract: Abstract: Silk hydrogels for tissue repair are usually pre-formed via chemical or physical treatments from silk solutions. For many medical applications, it is desirable to utilize injectable silk hydrogels at high concentrations (>8%) to avoid surgical implantation and to achieve slow in vivo degradation of the gel. In the present study, injectable silk solutions that formed hydrogels in situ were generated by mixing silk with low-molecular-weight polyethylene glycol (PEG), especially PEG300 and 400 (molecular weight 300 and 400 g mol −1 ). Gelation time was dependent on the concentration and molecular weight of PEG. When the concentration of PEG in the gel reached 40–45%, gelation time was less than 30 min, as revealed by measurements of optical density and rheological studies, with kinetics of PEG400 faster than PEG300. Gelation was accompanied by structural changes in silk, leading to the conversion from random coil in solution to crystalline β-sheets in the gels, based on circular dichroism, attenuated total reflection Fourier transform infrared spectroscopy and X-ray diffraction. The modulus (127.5 kPa) and yield strength (11.5 kPa) determined were comparable to those of sonication-induced hydrogels at the same concentrations of silk. The time-dependent injectability of 15% PEG–silk hydrogel through 27 G needles showed a gradual increase of compression forces from ∼10 to 50 N within 60 min. The growth of human mesenchymal stem cells on the PEG–silk hydrogels was hindered, likely due to the presence of PEG, which grew after a 5 day delay, presumably while the PEG solubilized away from the gel. When 5% PEG–silk hydrogel was subcutaneously injected in rats, significant degradation and tissue in-growth took place after 20 days, as revealed by ultrasound imaging and histological analysis. No significant inflammation around the gel was observed. The features of injectability, slow degradation and low initial cell attachment suggests that these PEG–silk hydrogels are of interest for many biomedical applications, such as anti-fouling and anti-adhesion.