Genotypic Determination of HIV Tropism in a Cohort of Patients Perinatally Infected With HIV-1 and Exposed to Antiretroviral Therapy. Issue 1 (January 2014)

• Record Type: Journal Article
• Authors: Di Biagio, Antonio; Parisini, Andrea; Bruzzone, Bianca; Prinapori, Roberta; Lauriola, Marinella; Paolucci, Stefania; Signori, Alessio; Barresi, Renata; Icardi, Giancarlo; Calderisi, Silvia; Meini, Genny; Dentone, Chiara; Cenderello, Giovanni; Guerra, Michele; Maccabruni, Anna; Rusconi, Stefano; Viscoli, Claudio
• Is Part Of: HIV clinical trials. Volume 15:Issue 1(2014)
• Page Start: 45
• Page End: 50
• Publication Date: 2014-01
• Subjects: adolescents, -- CCR5 antagonists, -- children, -- coreceptor tropism, -- HIV-1 DNA, -- V3 genotyping, -- vertical transmission; HIV Infections -- Chemotherapy -- Periodicals; AIDS (Disease) -- Chemotherapy -- Periodicals; HIV Infections -- Research -- Periodicals; AIDS (Disease) -- Research -- Periodicals; 616.979206105
• DOI: 10.1310/hct1501-45
• Languages: English
• ISSNs: 1528-4336

Abstract

Abstract : The aim of this study was to determine the coreceptor tropism by performing genotypic HIV-1 tropism testing in a cohort of patients perinatally infected with HIV-1 and exposed to antiretroviral therapy. Genotypic coreceptor tropism was determined in patients with HIV-1 RNA<100 copies/mL using PBMC samples by gp120 V3 sequencing followed by geno2pheno interpretation (set at a false positive rate [FPR] of 20%) and in patients with >100 copies/mL using plasma samples (set at a FPR of 20%), according to European guidelines. Out of 55 patients, 50 had an HIV-1 subtype B strain, and mean ( SD ) age was 18.2 (4.6) years. The median duration of antiretroviral therapy was 13 years (range, 3–23). Thirty-three (60%) patients harbored the R5 virus. At the time of the testing, the median CD4+ T lymphocyte cell count and percentage were 705 cells/mm 3 (474–905) and 32.5% in group R5 and 626 cells/mm 3 (450–755) and 31.7% in group X4/D-M, respectively. The nadir of CD4+ T-cell count in groups R5 and X4/D-M were 322 cells/mm 3 (230–427) and 340 cells/mm 3 (242–356), respectively. These differences were not statistically significant. Fifteen patients had HIV-1 RNA >50 copies/mL. The median HIV-1 RNA and HIV-1 DNA were comparable in both groups without a statistical difference. The study provides an overview of the prevalence of coreceptor tropism in a cohort of patients who were vertically infected with HIV-1. The high prevalence of X4/D-M-tropic strains may simply reflect the long-term exposure to HIV.