Trehalose is not a universal solution for solid lipid nanoparticles freeze-drying. (7th October 2013)

• Record Type: Journal Article
• Authors: Doktorovova, Slavomira; Shegokar, Ranjita; Fernandes, Lisete; Martins-Lopes, Paula; Silva, Amélia M.; Müller, Rainer H.; Souto, Eliana B.
• Is Part Of: Pharmaceutical development and technology. Volume 19:Number 8(2014)
• Page Start: 922
• Page End: 929
• Publication Date: 2013-10-07
• Subjects: Cryoprotectants -- shelf life -- solid lipid nanoparticles -- stability; Drug delivery systems -- Periodicals; Pharmaceutical technology -- Periodicals; Drugs -- Administration -- Research -- Periodicals; Drug Delivery Systems -- Periodicals; Pharmaceutical Preparations -- Periodicals; Technology, Pharmaceutical -- Periodicals; 615
• DOI: 10.3109/10837450.2013.840846
• Languages: English
• ISSNs: 1083-7450

Abstract

Abstract: Objective : To prepare stable and easy to handle formulation of solid lipid nanoparticles (SLNs) by freeze-drying with or without cryoprotectants, as appropriate. Materials and methods : SLNs were freeze-dried without cryoprotectants or with cryoprotectants in quantities selected by freeze–thaw test (sucrose, glucose) or literature search (trehalose, maltose). Appearance, re-dispersability and size distribution of re-dispersed samples were evaluated. Results : SLN could be freeze-dried using 10% sucrose, trehalose or maltose. Trehalose was effective in protecting one of presented formulations that was already very stable on its own; its efficiency in protecting other two formulations was limited. Discussion : Our results are in line with various reports of successful freeze-drying of SLN, but considering the stability of original dispersions, no improvement was achieved. Conclusion : We confirmed that trehalose is among the most suitable cryoprotectant for SLN, however it did not improve shelf-life of the most stable formulation.