Fluorine-18-deoxyglucose positron emission tomography/computed tomography with Ki67 and GLUT-1 immunohistochemistry for evaluation of the radiosensitization effect of oleanolic acid on C6 rat gliomas. Issue 1 (January 2015)

• Record Type: Journal Article
• Authors: Zhang, Yu; Xu, Huiqin; Wang, Hui; Yu, Wenjing; Zhao, Xuefeng; Xue, Yangyang
• Is Part Of: Nuclear medicine communications. Volume 36:Issue 1(2015:Jan.)
• Publication Date: 2015-01
• Subjects: C6 rat gliomas -- 18F-FDG PET/CT -- GLUT-1 -- Ki67 -- oleanolic acid; Nuclear medicine -- Periodicals; 616.07575
• DOI: 10.1097/MNM.0000000000000211
• Languages: English
• ISSNs: 0143-3636

Abstract

Abstract : Objective: The aim of this study was to investigate the radiosensitization effect of oleanolic acid (OA) in an in-vivo C6 rat glioma model using fluorine-18-deoxyglucose PET/computed tomography ( 18 F-FDG PET/CT) and Ki67 and glucose transporter-1 (GLUT-1) immunohistochemistry (IHC) and evaluate the utility of 18 F-FDG PET/CT in assessing early changes after radiotherapy. Materials and methods: Tumor-bearing rats were divided into four groups: the control group (group A), the OA group (group B), the radiotherapy group (group C), and the OA combined with radiotherapy group (group D). 18 F-FDG PET/CT images were obtained to monitor the tumor/muscle (T/M) ratio of 18 F-FDG uptake before treatment, 1 day after treatment, and 7 days after treatment. Tumor volume changes were also assessed, and hematoxylin and eosin staining and Ki67 and GLUT-1 IHC staining were also carried out. Results: Before treatment, there were no obvious differences between the T/M ratios ( F =0.147, P =0.931) and tumor volumes ( F =0.177, P =0.911) among the four groups. At day 1 after treatment, statistical differences were observed in the T/M ratios ( F =2.891, P =0.05), with decreased values in groups C and D compared with group A ( t CA =2.354, t DA =2.356, P <0.05). At day 7 after treatment, the T/M values of group D were lower than those of the other three groups ( t DC =2.416, P =0.03; t DA =8.133, t DB =7.656, P <0.01), and the tumor volumes of group D were significantly decreased compared with group C ( t =2.351, P =0.02). There were significant differences in tumor cell densities and in the expression of Ki67 and GLUT-1 in the four groups ( F =16.667, 22.082, and 39.555, P <0.001). The T/M ratio showed a significant correlation with the expression of Ki67 and GLUT-1, and our study also found a significant relationship between the expression of Ki67 and the expression of GLUT-1. Conclusion: OA has a radiosensitization effect on C6 rat glioma tumors in vivo, detected using 18 F-FDG PET/CT and Ki67 and GLUT-1 IHC staining. 18 F-FDG PET/CT is a potentially sensitive tool for the evaluation of early changes after radiotherapy.