Linear-after-the-exponential (LATE)-PCR: Improved asymmetric PCR for quantitative DNA-analysis. (December 2015)

• Record Type: Journal Article
• Authors: Schulze Johann, K.; Schürenkamp, M.; Sibbing, U.; Lischka, C.; Pfeiffer, H.; Vennemann, M.
• Is Part Of: Forensic science international. Volume 5(2015)
• Page Start: e659
• Page End: e661
• Publication Date: 2015-12
• Subjects: Forensic genetics -- Periodicals; Forensic Genetics -- Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.fsigss.2015.09.246
• Languages: English
• ISSNs: 1875-1768

Abstract

Abstract: Linear-after-the-exponential (LATE)-PCR describes a novel approach to asymmetric PCR which uses adjusted melting temperatures of the limited primer to increase PCR efficiency. In this proof-of-principle study we show that linear amplification is possible over a wide range of amplification cycles. The curve characteristics of the real time PCR show that the plateau effect of conventional PCR can be avoided by this innovative technique.