Bamboo-like multiwalled carbon nanotubes dispersed in double stranded calf-thymus DNA as a new analytical platform for building layer-by-layer based biosensors. (10th November 2015)

• Record Type: Journal Article
• Authors: Primo, Emiliano N.; Gutierrez, Fabiana A.; Rubianes, María D.; Rivas, Gustavo A.
• Is Part Of: Electrochimica acta. Volume 182(2015)
• Page Start: 391
• Page End: 397
• Publication Date: 2015-11-10
• Subjects: Carbon nanotubes functionalization -- calf-thymus double stranded DNA -- Glucose Oxidase -- Layer-by-layer -- Self-assembly -- Electrochemical glucose biosensor -- Bamboo-like carbon nanotubes; Electrochemistry -- Periodicals; Electrochemistry, Industrial -- Periodicals; 541.37
• DOI: 10.1016/j.electacta.2015.09.028
• Languages: English
• ISSNs: 0013-4686

Abstract

Graphical abstract: Highlights: bCNT-dsDNA is an efficient platform to build biosensors by LbL self-assembling of multilayers. GOx and PDDA were efficiently LbL self-assembled at GCE/bCNT-dsDNA. The interpenetration of the layers increases with the number of PDDA-GOx bilayers. The assembly of PDDA and GOx was efficient up to the third PDDA/GOx bilayer. GCE/bCNT-dsDNA/(PDDA/GOx)3 showed the greatest sensitivity, selectivity and stability. GCE/bCNT-dsDNA/(PDDA/GOx)3 was successfully used to quantify glucose in beverages and pharmaceutical samples. ABSTRACT: This work reports the successful application of bamboo-like multiwalled carbon nanotubes (bCNT) non-covalently functionalized with calf-thymus double stranded DNA (dsDNA) as a robust platform (bCNT-dsDNA) to build electrochemical biosensors. The "model system" proposed here as a proof of concept was an enzymatic biosensor devoted to glucose quantification obtained by layer-by-layer self-assemby of polydiallyldimethylammonium (PDDA) and glucose oxidase (GOx) at glassy carbon electrodes (GCE) modified with bCNT-dsDNA (GCE/bCNT-dsDNA/(PDDA/GOx)n ). The influence of GOx and PDDA assembling conditions and the effect of the number of PDDA/GOx bilayers (n) on the performance of the resulting biosensor is critically discussed. The supramolecular architecture was characterized by electrochemical impedance spectroscopy from the charge transfer resistance of quinone/hydroquinone and potassium ferrocyanide/potassium ferricyanide; by cyclic voltammetry from the surface concentration of GOx using ferrocene methanol as enzyme regenerator; by amperometry from the response of the enzymatically generated hydrogen peroxide; and by surface plasmon resonance from the changes in the plasmon resonance angle. The analytical parameters obtained with GCE/bCNT-dsDNA/(PDDA/GOx)3 for the amperometric quantification of glucose at 0.700 V were: sensitivity of (265 ± 7) μA mM −1 cm −2, linear range between 0.25 and 2.50 × 10 −3 M, detection limit of 50 μM, repeatability of 3.6% (n = 10), and negligible interference from maltose, galactose, fructose and manose. The biosensor was successfully used for the sensitive quantification of glucose in beverages and a medicine sample.