A flow‐through column electrolytic cell for supercritical fluid chromatography*. Issue 20 (12th September 2017)

• Record Type: Journal Article
• Authors: Yamamoto, Kazuhiro; Ueki, Tatsuya; Higuchi, Naoyuki; Takahashi, Kouji; Kotani, Akira; Hakamata, Hideki
• Is Part Of: Journal of separation science. Volume 40:Issue 20(2017)
• Page Start: 4085
• Page End: 4090
• Publication Date: 2017-09-12
• Subjects: electrochemical detection -- flow‐through column electrolytic cell -- supercritical fluid chromatography; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201700317
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract: A novel flow‐through column electrolytic cell was proposed as a detector to obtain current signals for supercritical fluid chromatography. The electrochemical cell consisted of two electrodes and its holder, and a working and a counter electrode were fabricated from 192 carbon strings, which were composed of 400 carbon fibers of 10 μm in diameter filled into a heat‐shrinkable tube. These electrodes were placed in the center of a holder made from polyether ether ketone blocks and they were separated by polytetrafluoroethylene membrane filters. To evaluate the sensitivity of this cell, a standard solution of ferrocene was injected into the supercritical fluid chromatography system connected to the electrolytic cell. The ferrocene was eluted through a silica gel column using a mixture of a mobile phase of supercritical CO2 and a modifier of methanol containing ammonium acetate. The current peak area of ferrocene correlated to the ferrocene concentration in the range of 10–400 μmol/L ( r = 0.999). Moreover, the limit of detection on the column estimated from a signal‐to‐noise ratio of 3 was 9.8 × 10 −13 mol.