Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation. (November 2017)

• Record Type: Journal Article
• Authors: Wang, Xinhong; Tang, Yinghua; Shen, Rui; Chen, Jirong; Chen, Guanying; Luo, Gang; Ge, Linhu
• Is Part Of: Cytokine. Volume 99(2017)
• Page Start: 275
• Page End: 280
• Publication Date: 2017-11
• Subjects: Oral ulcer -- Hepatocyte growth factor (HGF) -- Cytokine; Cytokines -- Periodicals; 571.844
• DOI: 10.1016/j.cyto.2017.08.006
• Languages: English
• ISSNs: 1043-4666

Abstract

Highlights: Influence of overexpression HGF on the healing of oral ulcer was evaluated. Serum cytokines were detected using Antibody arrays. HGF exhibited anti-inflammatory potential in oral traumatic ulcer of mice. Abstract: Objective: To evaluate the influence of overexpression HGF on the healing of traumatic ulcer of oral mucosa of mice. Material and methods: Mice were divided into two groups: wild type C57BL6(WT) and HGF high expression transgenic (HGF-Tg) mice. Traumatic ulcer of all mice were made by number 15 scalpel blade. Mice were sacrificed after 5 days and the inflammation score and expression of TNFα, IFNγ, c-Met, apoptosis (TUNEL) and 40 serum inflammation cytokines were estimated. Results: HGF-Tg mice presented a lower inflammation score (p = 0.011), Serum TNFα expression in HGF-Tg ulcers is 1.3 times than WT ulcer and the difference is statistical significance ( t test, p = 0.003). Serum c-Met protein in HGF-Tg mice were significantly higher than WT mice ( t test, p = 0.004). No statistical difference was observed in the serum IFNγ between WT ulcer and HGF-Tg ulcer ( t test, p = 0.268). TNFα positive cytoplasm expression cells in connective tissue of HGF-Tg mice is significantly lower than that of WT group ( t test, p = 0.029). C-Met positive cytoplasm expression cells in both epithelium and connective tissue of HGF-Tg group is significantly higher than that of WT group ( t test, p = 0.040, p = 0.000). Samples in HGF-Tg group showed a lower number of positive cells of epithelium TUNEL staining compared with that in the WT group ( t test, p = 0.035). Conclusions: HGF exhibited anti-inflammatory potential in oral traumatic ulcer through the reduction of epithelial apoptosis, connective tissue TNFα expression and induction of c-Met expression.