Biocompatible chitosan based hydrogels for potential application in local tumour therapy. (1st January 2018)

• Record Type: Journal Article
• Authors: Olaru, Anda-Mihaela; Marin, Luminita; Morariu, Simona; Pricope, Gabriela; Pinteala, Mariana; Tartau-Mititelu, Liliana
• Is Part Of: Carbohydrate polymers. Volume 179(2018)
• Page Start: 59
• Page End: 70
• Publication Date: 2018-01-01
• Subjects: Chitosan -- Nitrosalicylaldehyde -- Hydrogel -- Biocompatibility -- Antitumor; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.09.066
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: Hydrogels based on chitosan and nitrosalicyladehyde were synthesized via DCC chemistry. The hydrogels have superporous morphology, elastic properties and good recovery capacity. They swell very fast in different pH media and preserve their dimensions in basic medium. They present in vitro cytotoxicity on HeLa cells and in vivo biocompatibility in laboratory rats. Abstract: A series of hydrogels based on chitosan polyamine and nitrosalicylaldehyde were prepared via dynamic covalent chemistry (DCC), by imination and transimination reactions towards ordered clusters which play the role of crosslinking nodes of the chitosan network. The hydrogelation mechanism has been proved through NMR and FTIR spectroscopy, X-ray diffraction and polarized light microscopy. The successful preparation of the hydrogels and their mechanical properties were further investigated using rheological measurements. By electron scanning microscopy, the hydrogels exhibited a channels microstructure morphology which critically influenced their fast swelling by capillarity. The hydrogels cytotoxicity was explored in vitro on HeLa cancer cells and their biocompatibility was monitored in vivo by subcutaneous implantation on rats. The novel hydrogels proved good in vitro cytotoxicity on the HeLa cells and also in vivo biocompatibility in rats. Thus, these novel biomaterials promise to be suitable for local cancer therapy.