Antimicrobial activity of catechol functionalized-chitosan versus Staphylococcus epidermidis. (1st January 2018)

• Record Type: Journal Article
• Authors: Amato, Andrea; Migneco, Luisa Maria; Martinelli, Andrea; Pietrelli, Loris; Piozzi, Antonella; Francolini, Iolanda
• Is Part Of: Carbohydrate polymers. Volume 179(2018)
• Page Start: 273
• Page End: 281
• Publication Date: 2018-01-01
• Subjects: Chitosan -- Hydrocaffeic acid -- Bioadhesive polymers -- Protein mussels mimics polymers -- Wound dressings; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.09.073
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: New protein mussels mimicschitosan (CS) derivatives have been synthesized by reaction with hydrocaffeic acid (HCAF). An efficient procedure for acetonide-protection of the catechol moiety of HCAF was set up. The CS-HCAF conjugates showed good antioxidant activity confirming the efficacy of the adopted synthetic strategy. Chitosan derivatives possessed enhanced antimicrobial activity versus Staphylococcus epidermidis compared to pure chitosan. Commercial dressing coated with a CS-HCAF conjugate was able to reduce S. epidermidis adhesion and prevent biofilm formation. Abstract: Protein mussel-inspired adhesive polymers, characterized by the presence of catechol groups, possess superior muco-adhesive properties and have great potentiality in wound healing. Suitable materials for wound dressing should properly combine muco-adhesiveness and antimicrobial activity. In this work, catechol-functionalized chitosan was obtained by reaction with hydrocaffeic acid (HCAF), in order to investigate how catechol introduction at different content could affect the intrinsic antimicrobial activity of the polymer itself. Unexpectedly, an enhancement of chitosan antimicrobial activity was observed after catechol functionalization, with a fourfold reduction in the polymer minimum inhibitory concentration versus Staphylococcus epidermidis . Additionally, a commercial wound dressing coated with one of the synthesized CS-HCAF derivatives showed a significant reduction in the adhesion of S. epidermidis compared to the uncoated dressing (3-log reduction). The CS-HCAF derivatives also showed an interesting antioxidant property (EC50 ranging from 20 to 60 μg/mL), which further confirms the potentiality of these materials as wound dressings.