Antioxidant, anti-rheumatic and anti-inflammatory investigation of extract and dicentrinone from Duguetia furfuracea (A. St.-Hil.) Benth. & Hook. f. (30th January 2018)

• Record Type: Journal Article
• Authors: do Santos, Rafael Claudino; de Souza, Alexsandra Villamaior; Andrade-Silva, Magaiver; Vera Cruz, karoliny Cardoso; Leite Kassuya, Candida Aparecida; Lima Cardoso, Claúdia Andréa; Vieira, Maria do Carmo; Nazari Formagio, Anelise Samara
• Is Part Of: Journal of ethnopharmacology. Volume 211(2017)
• Page Start: 9
• Page End: 16
• Publication Date: 2018-01-30
• Subjects: MEDF methanolic extract of Duguetia Furfuracea -- CF chloroform fraction -- EAF ethyl acetate fraction -- HMF hydromethanol fraction -- DF-1 dicentrinone -- LC-PDA high-performance liquid chromatography coupled with photodiode array detection -- DPPH 2, 2-Diphenyl-1-picrylhydrazyl -- ABTS 2, 2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) -- MDA malondialdehyde -- BHT butylated hydroxytoluene -- PBS phosphate buffered saline -- 1H NMR proton nuclear magnetic resonance -- 13C NMR carbon-13 nuclear magnetic resonance -- TMS tetramethylsilane -- MHz megahertz -- CD3OD Deuterated methanol -- MeOH methanol -- H2O water -- H2SO4 sulfuric acid -- TLC thin-layer chromatography -- CC column chromatography -- CHCl3 chloroform-D -- LC liquid chromatography -- PDA photodiode array -- MS mass spectroscopy -- ESI electrospray ionization -- GAE gallic acid equivalent -- nm nanometer -- min minute -- QE quercetin equivalent -- AlCl3 aluminum chloride -- h hour -- DEX dexamethasone -- EDTA Ethylenediamine tetraacetic acid -- HPLC-DAD high-performance liquid chromatography coupled with diode-array detector -- A0 initial absorbance -- A absorbance -- IC50 half maximal inhibitory concentration -- I% inhibition percent -- AA antioxidant activity -- DNA deoxyribonucleic acid -- NSAIDs non-steroidal anti-inflammatory drugs; Araticum-seco -- Inflammation -- Mice -- Alkaloid -- Antioxidant -- Dicentrinone; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2017.09.019
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: The preparations of teas and syrups using Duguetia furfuracea have been used in folk medicine to treat rheumatism and back pain. Several rheumatic diseases are anti-inflammatory and are treated with several anti-inflammatories. Aim of the study: The objective of this work were to evaluate the chemical investigation of methanolic extract obtained from leaves of D. furfuracea (MEDF) and test the MEDF, such as chloroform (CF), ethyl acetate (EAF) and hydromethanol (HMF) fractions and the alkaloid dicentrinone (DF-1) in vitro antioxidant effects and in vivo models of inflammation. Material and methods: MEDF and CF were analyzed by LC-PDA and the results revealed the presence of alkaloid aporphine and oxoaporphine. The concentrations of total phenols, flavonoids and flavonols were determined. Additionally, MEDF, fractions and dicentrinone were evaluated free radical scavenging activity 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and peroxidation β-carotene/linoleic acid and malondialdehyde (MDA) assays. The anti-inflammatory effects of MEDF, fractions and dicentrinone were studied in carrageenan-induced paw edema. The anti-rheumatic potential was studied in air pouch model and zymosan-induced arthritis. Results: CF fractionation resulted in the isolation of the oxaporphine alkaloid dicentrinone (DF-1). The highest phenols (624.37 mg GAE/g extract), flavonoids (580.51 mg QE/g extract) and flavonols (254.44 mg QE/g extract), concentration was found in extract. In antioxidant tests, MEDF exhibited the highest scavenging activity and lipoperoxidation. The extract (30–300 mg/kg) and all tested fractions inhibited the edema induced by carrageenan. The oral administration of DF-1 inhibited both edema associated with carrageenan-induced inflammation in mice. In air pouch model of inflammation, MEDF (30–300 mg/kg) and DF-1 (100 mg/kg) inhibited leukocyte migration and plasmatic leakage induced by carrageenan in mice. Finally, MEDF (100 mg/kg) did not alter zymozan-induced arthritis in mice. Conclusion: The results showed that D. furfuracea exhibits antioxidant, anti-rheumatic potential and anti-inflammatory activity. The presence of the alkaloid dicentrinone in extract and CF fraction could be responsible, at least in part, for the observed effects. Graphical abstract: