Characterization of SCCmec and spa types of methicillin-resistant Staphylococcus aureus isolates from health-care and community-acquired infections in Kerman, Iran. Issue 4 (December 2017)

• Record Type: Journal Article
• Authors: Fasihi, Yaser; Kiaei, Somayeh; Kalantar-Neyestanaki, Davood
• Is Part Of: Journal of epidemiology and global health. Volume 7:Issue 4(2017:Dec.)
• Page Start: 263
• Page End: 267
• Publication Date: 2017-12
• Subjects: MRSA -- VRSA -- SCCmec -- spa type; Epidemiology -- Periodicals; World health -- Periodicals; Public Health -- Periodicals; Communicable Disease Control -- Periodicals; World Health -- Periodicals; Electronic journals; 614.405
• DOI: 10.1016/j.jegh.2017.08.004
• Languages: English
• ISSNs: 2210-6014

Abstract

Highlights: The prevalence of MRSA in the hospitals and community is high. The emergence of VRSA isolates could be caused to decrease the therapeutic options. The more strict strategies are needed for limiting the dissemination of MRSA. Abstract: Spread of methicillin-resistant Staphylococcus aureus (MRSA) isolates is a worldwide problem. Molecular typing is a useful tool to understand MRSA epidemiology. Herein, we determined vancomycin-resistant, SCC mec and spa types among MRSA isolates recovered from healthcare and community-acquired infections in Kerman, Iran. A total of 170 S. aureus isolates were collected from different patients who were admitted to affiliated hospitals of Kerman University of Medical science. MRSA and vancomycin-resistant S. aureus (VRSA) isolates were detected by phenotypic methods. Polymerase chain reaction (PCR) technique was used for detection of mecA, vanA and vanB genes. Staphylococcal cassette chromosomemec (SCC mec ) and spa typing were used for molecular typing of among MRSA isolates. Overall, 53% of isolates were considered as MRSA. Two MRSA isolates were resistant to vancomycin and vanA was detected in only one of VRSA isolates. SCC mec type III belonged to spa types t030 and t459 which they were the dominant spa types among community-associated MRSA (CA-MRSA) and healthcare-acquired MRSA (HA-MRSA) isolates. Our findings showed that the SCC mec type I and III spread from hospital settings to community, although the SCC mec type IV spread from community to healthcare systems. We have also reported VRSA isolates from hospitalized patients, therefore, appropriate policies should be enforced in order to prevent the spread of antibiotic resistance isolates in hospitals settings.