A novel synthetic chemistry approach to linkage-specific ubiquitin conjugation. Issue 14 (4th March 2015)

• Record Type: Journal Article
• Authors: Morgan, Rachel E.; Chudasama, Vijay; Moody, Paul; Smith, Mark E. B.; Caddick, Stephen
• Is Part Of: Organic & biomolecular chemistry. Volume 13:Issue 14(2015)
• Page Start: 4165
• Page End: 4168
• Publication Date: 2015-03-04
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c5ob00130g
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : Site-specific ubiquitin cysteine mutants enable an elegant method for the linkage-specific conjugation of ubiquitins through dibromomaleimides and dibromopyrdazinediones. Abstract : Ubiquitination is of great importance as the post-translational modification of proteins with ubiquitin, or ubiquitin chains, facilitates a number of vital cellular processes. Herein we present a facile method of preparing various ubiquitin conjugates under mild conditions using michael acceptors based on dibromo-maleimides and dibromo-pyridazinediones.