Neuronal Alignment and Outgrowth on Microwrinkled Conducting Polymer Substrates. Issue 1795 (2015)

• Record Type: Journal Article
• Authors: Bonisoli, Alberto; Marino, Attilio; Ciofani, Gianni; Greco, Francesco
• Editors: Rivnay, J.; Bettinger, C.; Ng, T.N.; Wallace, G.G.
• Is Part Of: MRS proceedings. Issue 1795(2015)
• Page Start: 13
• Page End: 18
• Publication Date: 2015
• Subjects: biomedical, -- nanostructure, -- self-assembly; Electrical engineering -- Congresses; Physics -- Congresses; Materials -- Research -- Congresses; Materials science -- Congresses; 620.11
• DOI: 10.1557/opl.2015.558
• Languages: English
• ISSNs: 0272-9172

Abstract

ABSTRACT: We report on the results of culturing SH-SY5Y neuron-like cells on PEDOT:PSS wrinkled surfaces fabricated by thermally-induced shrinking of commercial polystyrene sheets. Such smart biointerfaces combine the functional properties of conducting polymers with the topographic patterning at the micro- and sub-microscale, as a result of surface wrinkling. By imposing mechanical constraints during shrinking, anisotropic topographic features are formed, with a spatial periodicity in the range 0.7 - 1.2 μm, tunable by varying the thickness of the PEDOT:PSS thin film. The effectiveness of wrinkled surfaces in enhancing and orientating the outgrowth of neurites is demonstrated by a 42% increase in length and by the 85% of neurites aligned along wrinkles direction (angle 0 < θ < 15°), after 5 days of differentiation. Furthermore, the conductive properties of the PEDOT:PSS film are retained after the surface wrinkling, opening the way for the exploitation of these smart biointerfaces for the electrical stimulation of cells.