Deficiency of Superoxide Dismutase Impairs Protein C Activation and Enhances Susceptibility to Experimental Thrombosis. Issue 8 (August 2015)

• Record Type: Journal Article
• Authors: Dayal, Sanjana; Gu, Sean X.; Hutchins, Ryan D.; Wilson, Katina M.; Wang, Yi; Fu, Xiaoyun; Lentz, Steven R.
• Is Part Of: Arteriosclerosis, thrombosis, and vascular biology. Volume 35:Issue 8(2015)
• Publication Date: 2015-08
• Subjects: catalase -- protein C -- superoxides -- thrombomodulin -- thrombosis; Arteriosclerosis -- Periodicals; Thrombosis -- Periodicals; Blood-vessels -- Pathophysiology -- Periodicals; Electronic journals; 616.13
• DOI: 10.1161/ATVBAHA.115.305963
• Languages: English
• ISSNs: 1079-5642

Abstract

Abstract : Objective—: Clinical evidence suggests an association between oxidative stress and vascular disease, and in vitro studies have demonstrated that reactive oxygen species can have prothrombotic effects on vascular and blood cells. It remains unclear, however, whether elevated levels of reactive oxygen species accelerate susceptibility to experimental thrombosis in vivo. Approach and Results—: Using a murine model with genetic deficiency in superoxide dismutase-1 (SOD1), we measured susceptibility to carotid artery thrombosis in response to photochemical injury. We found that SOD1-deficient ( Sod1 −/− ) mice formed stable arterial occlusions significantly faster than wild-type ( Sod1 +/+ ) mice ( P <0.05). Sod1 −/− mice also developed significantly larger venous thrombi than Sod1 +/+ mice after inferior vena cava ligation ( P <0.05). Activation of protein C by thrombin in lung was diminished in Sod1 −/− mice ( P <0.05 versus Sod1 +/+ mice), and generation of activated protein C in response to infusion of thrombin in vivo was decreased in Sod1 −/− mice ( P <0.05 versus Sod1 +/+ mice). SOD1 deficiency had no effect on the expression of thrombomodulin, endothelial protein C receptor, or tissue factor in lung or levels of protein C in plasma. Exposure of human thrombomodulin to superoxide in vitro caused oxidation of multiple methionine residues, including critical methionine 388, and a 40% decrease in thrombomodulin-dependent activation of protein C ( P <0.05). SOD and catalase protected against superoxide-induced methionine oxidation and restored protein C activation in vitro ( P <0.05). Conclusions—: SOD prevents thrombomodulin methionine oxidation, promotes protein C activation, and protects against arterial and venous thrombosis in mice. Abstract : Supplemental Digital Content is available in the text.