Programmable Mg2+-dependent DNAzyme switch by the catalytic hairpin DNA assembly for dual-signal amplification toward homogeneous analysis of protein and DNA. Issue 34 (30th March 2015)

• Record Type: Journal Article
• Authors: Liu, Shufeng; Cheng, Chuanbin; Gong, Hongwei; Wang, Li
• Is Part Of: Chemical communications. Volume 51:Issue 34(2015)
• Page Start: 7364
• Page End: 7367
• Publication Date: 2015-03-30
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c5cc01649e
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : The catalytic hairpin DNA assembly-programmed Mg 2+ -dependent DNAzyme switch was proposed for dual-signal amplified detection of protein and DNA. Abstract : The catalytic hairpin DNA assembly-programmed active Mg 2+ -dependent DNAzyme was proposed for dual-signal amplified detection toward protein and DNA. The protein detection was implemented with the further combination of an additional terminal protection strategy. The detection limit toward avidin and target DNA could be achieved as 2 pM and 0.5 pM, respectively, with a high selectivity.