A two-photon fluorescent probe for detecting endogenous hypochlorite in living cells. Issue 14 (11th March 2015)

• Record Type: Journal Article
• Authors: Wang, Xunming; Wang, Xiaohui; Feng, Yan; Zhu, Manzhou; Yin, Hang; Guo, QingXiang; Meng, Xiangming
• Is Part Of: Dalton transactions. Volume 44:Issue 14(2015)
• Page Start: 6613
• Page End: 6619
• Publication Date: 2015-03-11
• Subjects: Chemistry, Inorganic -- Periodicals; Chemistry, Physical and theoretical -- Periodicals; Chemistry, Inorganic -- Periodicals; 546.05
• DOI: 10.1039/c5dt00012b
• Languages: English
• ISSNs: 1477-9226

Abstract

Abstract : A highly selective two-photon fluorescent probe (HQ ) for endogenous hypochlorite (ClO − ) was developed. Abstract : A novel two-photon fluorescent probe (HQ ) for hypochlorite (ClO − ) has been developed based on specific ClO − -promoted oxidation of a CN bond. The probe shows linear fluorescence responses to ClO − with 23.5-fold enhancement. Two-photon fluorescence detection signals ofHQ to ClO − were observed with high selectivity and sensitivity. The good biocompatibility guaranteed the use ofHQ to detect intracellular ClO − under two-photon excitation. Moreover, HQ has been successfully applied to monitor the endogenously produced hypochlorite in living cells (BV-2 ) stimulated by lipopolysaccharide (LPS).HQ provides a new ClO − detection agent for the discovery of pharmacological microglia modulators by high-throughput screening.