Rational design of a photo-crosslinking BODIPY for in situ protein labeling. Issue 30 (17th March 2015)

• Record Type: Journal Article
• Authors: Murale, Dhiraj P.; Hong, Seong Cheol; Yun, JiHyeon; Yoon, Chang No; Lee, Jun-Seok
• Is Part Of: Chemical communications. Volume 51:Issue 30(2015)
• Page Start: 6643
• Page End: 6646
• Publication Date: 2015-03-17
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c5cc01068c
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : We report the first rational design of a photo-crosslinking BODIPY fluorophore (pcBD ) and its biological application for biomolecule labeling in a spatiotemporally controlled manner. Abstract : Photo-crosslinking agents have emerged as critical tools to investigate protein–protein interactions in complex proteomes, but there are few photocrosslinkers available at the moment. Here, we report the first rational design of a photo-crosslinking BODIPY fluorophore (pcBD ) and its biological application for biomolecule labeling. As a photosensitizing functional motif, an aryl ketone group was incorporated into the BODIPY fluorophore, and a series of proteins were labeled bypcBD compounds upon UV irradiation. In order to investigate protein–protein interactions in a protein mixture, amino-functionalizedpcBD was prepared and covalently attached to a ubiquitin ligase binding peptide. Upon UV irradiation, we could successfully visualize the substrates in the total lysate. These results provided a proof of concept for spatially controllable tagging via photo-activation of thepcBD scaffold, and demonstrated its potential usage for in situ labeling applications.